Abstract
The authors develop an approach to reconstruct continuous energy and force landscapes over large distances accurate even at points of high force gradients.
Highlights
As chemical, physical, and biological interactions are all governed by their interaction energy landscapes [1,2], experimental methods to probe the underlying energy landscape are essential to understand fundamental interactions
Optical tweezer (OT) based single-molecule force spectroscopy pioneered the experimental reconstruction of the energy landscape of an interaction field using the Boltzmann based method to define DNA and RNA folding interactions [3,4,5]
In this Letter, we developed a framework to define characteristic forces to serve as reliable fiduciary markers of regions of the force curve that are typically undersampled in adhesive interactions
Summary
Physical, and biological interactions are all governed by their interaction energy landscapes [1,2], experimental methods to probe the underlying energy landscape are essential to understand fundamental interactions. Optical tweezer (OT) based single-molecule force spectroscopy pioneered the experimental reconstruction of the energy landscape of an interaction field using the Boltzmann based method to define DNA and RNA folding interactions [3,4,5]. In contrast to folding energy landscapes, interfacial interactions are typically stronger, the well gradients are larger, which requires higher sampling in these regions and a stiffer force probe. In the case of adhesive and interfacial interactions, there do not currently exist natural fiduciary markers by which multiple force curves can be combined.
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