Abstract
The trans-Golgi network (TGN) is an important cargo sorting station in the secretory pathway. To reveal insights into protein sorting at the TGN, it is important to develop an assay to quantify the efficiency of cargo capture. Here, we describe an experimental approach to reconstitute the packaging of cargo proteins into vesicles at the TGN in vitro. We also describe an experimental approach to immunoisolate vesicles enriched with a specific transmembrane cargo client from the in vitro vesicle formation assay. These assays provide robust tools to directly measure the enrichment of cargo proteins into TGN-derived vesicles and to reveal novel factors that regulate TGN export process.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
