Abstract
The heterologous gene expression system with Aspergillus oryzae as the host is an effective method to investigate fungal secondary metabolite biosynthetic pathways for reconstruction to produce un-natural molecules due to its high productivity and genetic tractability. In this review, we focus on biosynthetic studies of fungal polyketide-derived meroterpenoids, a group of bioactive natural products, by means of the A. oryzae heterologous expression system. The heterologous expression methods and the biosynthetic reactions are described in detail for future prospects to create un-natural molecules via biosynthetic re-design.
Highlights
Aspergillus oryzae is a fungus that has been utilized for over 2000 years in the Japanese fermentation industry to yield sake, miso, and soy sauce, and for industrial enzyme production
Aspergillus species that produce numerous secondary metabolites, such as mycotoxins, aflatoxin, medicinal compounds, lovastatin, and pigments such as emodin [1,2,3], as well as other secondary metabolite-producing fungi. For investigations on their biosynthesis, the heterologous expression system is a valuable tool, as it provides information about their reactions based on the products accumulated in the transformants
The A. oryzae genome includes some genes encoding secondary metabolite biosynthetic enzymes, such as the non-ribosomal peptide synthetase, which is responsible for the production of toxic compounds, such as cyclopiazonic acid
Summary
Aspergillus oryzae is a fungus that has been utilized for over 2000 years in the Japanese fermentation industry to yield sake, miso, and soy sauce, and for industrial enzyme production. Aspergillus species that produce numerous secondary metabolites, such as mycotoxins, aflatoxin, medicinal compounds, lovastatin, and pigments such as emodin [1,2,3], as well as other secondary metabolite-producing fungi For investigations on their biosynthesis, the heterologous expression system is a valuable tool, as it provides information about their reactions based on the products accumulated in the transformants. By using the versatile A. oryzae host, which synthesizes polyketide and terpenoid compoundswith withhigh highyields, yields,we wecan cancreate create aa fungal fungal meroterpenoid meroterpenoidproproandterpenoid terpenoidcompounds and duction system. This method includes important benefits, in that we can exploit the novel duction system.
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