Reconstitution of Photosystems I and II using spinach subchloroplast fragments fractionated by Triton treatment

Abstract

Reconstitution of Photosystems I and II was demonstrated using small (approx. 10 nm diameter) and photochemically highly active subchloroplast fragments fractionated from spinach by Triton treatment and using diphenylcarbazide as a Photosystem-II electron donor. NADP +-reduction activity of the reconstituted system was comparable to that of the unfractionated chloroplasts. Plastocyanin (or Euglena cytochrome 552) was an absolute requirement. Lecithin presumably served as a “binding agent”.