Abstract
Reconstitution of GPCRs in Lipidic Cubic Phase (LCP): Comparison between Human Histamine 1 and Dopamine 2 Long Receptor Reconstitution into Five Different Self-Assembly Lipids
Highlights
The success of in meso crystallization is the result of the lipidic cubic phase (LCP) introduction to the crystallization process
We have demonstrated that the bilayer thickness and water channel size of the self-assembly lipid affects the reconstitution of a G protein-coupled receptors (GPCRs) and this may affect which lipids to be used for Lipidic Cubic Phase (LCP) crystallization trials
The purified Dopamine 2 long receptor (D2L) receptor was reconstituted into five different self-assembly lipids; Monoolien (MO), Phytantriol (PT), Phytanoyl ethanomide (PE), H-farnesoyl monoethanolamide (FE) and Phytanyl Diethanolamide (PDiE) at two different concentrations of 3 and 5 mg/ml of receptor
Summary
The success of in meso crystallization is the result of the lipidic cubic phase (LCP) introduction to the crystallization process. LCP lipids are self-assembly lipids that have the unique properties and are closely related to the native lipid environment of a cell. They contain two domains which consist of the hydrophobic bilayer that accommodates the hydrophobic portion of the receptor and interconnecting water channels, which satisfy the hydrophilic portions of the receptor. There are two restrictions of the LCP environment that affects the uptake of the receptor so that it remains in a functional state; bilayer thickness and water channel diameter The size of these domains determine the lipids compatibility with the GPCR of interest and determines the phase of the lipid upon reconstitution in the initial phase of crystallization
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