Abstract
Cytochrome c oxidase vesicles prepared using enzyme preparations subjected to cycles of freezing and thawing (+20 to −20°C) before reconstitution, display a decrease in respiratory control ratio (RCR); if the protein is incubated with detergents before reconstitution, a higher RCR value is restored. This effect is attributed to a detergent-mediated optimization of the structural assembly of the proteo-membrane unit occurring at the early stages of reconstitution. The same type of experiment carried out at different temperatures showed that incubation at 35°C for 30 min leads to a severe, irreversible loss of RCR.
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