Abstract
Membrane vesicles from rat cerebellum were reconstituted into lipid bilayers. The activity of two different potassium channels was recorded: (1) a small conducting voltage dependent potassium channel insensitive to [Ca 2+] i, (2) a calcium and voltage dependent potassium channel (K Ca). K Ca channels had a conductance of (302±15) pS ( n=5) and were activated by [Ca 2+] i and membrane depolarizations. They were blocked by tetraethylamonium (TEA) and charybdotoxin (CTX) but insensitive to noxiustoxin (NTX). Finally, we showed the blocking effect of Androctonus australis Hector (AaH) scorpion venom on K Ca channels from rat cerebellum.
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