Abstract
Giant unilamellar vesicles (GUVs) are a great model system to explore the functional mechanisms of the constantly rearranging cytoskeletal network in a bottom-up approach. Here, we developed a reconstituted active actin network consisting of actin filaments, crosslinking proteins and myosin-II motor filaments in confinement. In this minimal model system, the key parameters are tightly controlled, which leads the way to gain a better understanding of the basic physical principles underlying the complex cytoskeletal dynamics. By means of quantitative fluorescence microscopy and image analysis, the interplay between force generation by molecular motors and the stabilization of the network by crosslinking proteins is identified to be responsible for the highly dynamic structure formation process.
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