Abstract
IntroductionAnisakis spp., during parasitism, release excretory-secretory antigens that, in contact with the human immune system, can trigger a hypersensitivity response mediated by IgE, causing various allergic symptoms. ObjectivesTo evaluate the IgE response in Wistar rats after infection with L3 larvae of the parasite Anisakis spp. MethodsSome determining factors involved in the technique have been improved in this work, such as: the concentration of polyacrylamide used in the preparation of the gels, the antigen concentration used, and the temperature required for denaturation of proteins. ResultsImmune responses (Ag-Ab) observed by the immunoblotting technique showed a greater intensity with serum obtained after reinfection, which have recognized proteins that may correspond to the major antigen Ani s 1 and other polypeptides of interest in the diagnosis of human anisakiasis. ConclusionThis paper concludes that immunoblotting is a useful technique to detect IgE antibodies against Anisakis proteins.
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