Reconfiguring droplet interface bilayer networks through sacrificial membranes.

Abstract

The droplet interface bilayer platform allows for the fabrication of stimuli-responsive microfluidic materials, using phospholipids as an organic surfactant in water-in-oil mixtures. In this approach, lipid-coated droplets are adhered together in arranged networks, forming lipid bilayer membranes with embedded transporters and establishing selective exchange pathways between neighboring aqueous subcompartments. The resulting material is a biologically inspired droplet-based material that exhibits emergent properties wherein different droplets accomplish different functions, similar to multicellular organisms. These networks have been successfully applied towards biomolecular sensing and energy harvesting applications. However, unlike their source of inspiration, these droplet structures are often static. This limitation not only renders the networks unable to adapt or modify their structure and function after formation but also limits their long term use as passive ionic exchange between neighboring droplet pairs may initiate immediately after the membranes are established. This work addresses this shortcoming by rupturing selected sacrificial membranes within the collections of droplets to rearrange the remaining droplets into new configurations, redirecting the droplet-droplet exchange pathways. This is accomplished through electrical shocks applied between selected droplets. Experimental outcomes are compared to predictions provided by a coupled mechanical-electrical model for the droplet networks, and then advanced configurations are proposed using this model.