Recombination Values for the Ms6‐W1 Chromosome Region in Different Genetic Backgrounds in Soybean

Abstract

Recombination values in plants are influenced by genetic factors, environmental conditions, and their interactions. In soybean, Glycine max (L.) Merr., the cosegregation method to produce hybrid seed depends on the close linkage of the Ms6(pollen fertility) locus and the W1 (flower color) locus. Twenty-four near-isogenic lines, cosegregating at the Ms6 and W1 loci, (Ms6W1 ms6w1 ), were developed with the ms6-w1 donor cytoplasm and the recurrent parent cytoplasms. The objectives were to determine the recombination values between the Ms6 and W1 loci from both testcross and F2-family data. The recombination value for the Ms6 and W1 loci from the testcross data in the ms6-w1 donor cytoplasm was 3.14 ± 0.80 and in the recurrent parent cytoplasms was 3.62 ± 0.89. From the F2- family data, the recombination value for the Ms6 and W1 loci in the ms6-w1 donor cytoplasm was 3.06 ± 0.35 and in the recurrent parent cytoplasms was 4.90 ± 0.35. The recombination values for the F2-family data were statistically significantly different (P = 0.05) for the ms6-w1 donor cytoplasm versus the recurrent parent cytoplasms. However, the recombination values are acceptable to continue to use the cosegregation method to produce hybrid soybean seed.