Recombination analysis of Human mastadenovirus C whole genomes

Pierre Rivailler,Zhen Zhu,Wenbo Xu,Naiying Mao

doi:10.1038/s41598-019-38719-z

Pierre Rivailler, Zhen Zhu + Show 2 more

Open Access

https://doi.org/10.1038/s41598-019-38719-z

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Abstract

This study aims at analyzing all publicly available HAdV-C whole genome sequences (WGSs) and describes the genetic relationships between these genomes as well as identifies potential hotspots for recombination throughout the viral genome. In addition to the 4 prototypical genomic sequences, this analysis identified 20 HAdV-C WGSs which should be relevant for future recombination analysis of HAdV-C. This report confirmed the recombinogenic property of HAdV-C genomes and identified two main regions for breakpoints, within the hexon gene and around the fiber genomic region. No obvious recombination was detected between HAdV-Cs and non-human mastadenoviruses or non-C HAdVs. Finally, it highlighted the need for a surveillance of HAdVs in order to detect novel recombinant types that might represent health risks and develop possible prevention measures. Genetic analyses of recombination between recently collected HAdV-Cs and the assessment of their potential virulence are necessary steps towards the establishment of a surveillance of HAdVs in the future.

Highlights

The human mastadenovirus (HAdV) is a non-enveloped, double-stranded DNA virus of the family Adenoviridae within the genus Mastadenovirus[1]
As multiple studies revealed that HAdV was prone to intratypic recombination, the 9th International Adenovirus Meeting proposed to use whole genome sequences (WGSs) to characterize and name novel HAdV types[4]
Our analysis identified several categories of sequences which should facilitate future recombination analyses of HAdV-C genomes

Summary

Introduction

The human mastadenovirus (HAdV) is a non-enveloped, double-stranded DNA virus of the family Adenoviridae within the genus Mastadenovirus[1]. HAdVs are divided into 7 species (HAdV-A to G) with 90 types (as of July 2018) based on biological properties, serum neutralization assays, and whole-genome sequencing analysis[3]. The HAdV working group established a nomenclature based on penton base, hexon and fiber sequences (PHF), and is continuously updating the nomenclature based on biological and genomics data The genomes HQ003817, (prototype of type 57) and KR699642 (strain CBJ113) were described in the context of the 4 prototype viruses collected in 1953 (type 1, 2, 5 and 6)[5,6]. This study aims at analyzing all publicly available HAdV-C WGSs and describes the genetic relationships between these genomes as well as identifies potential hotspots for recombination throughout the genome

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