Recombinant pseudorabies virus strains as intracranially injected retrograde tracers

Abstract

Event Abstract Back to Event Recombinant pseudorabies virus strains as intracranially injected retrograde tracers É. R. Szabó1, D. Tombácz2, Z. Boldogkői2, M. Palkovits1 and Á. Dobolyi1* 1 Hungarian Academy of Sciences and Semmelweis University, Department of Anatomy, Histology and Embryology, Hungary 2 University of Szeged, Department of Medical Biology, Hungary Recently developed recombinant neurotropic pseudorabies (PRV) viruses were pressure injected into the auditory cortex in rats. The medial geniculate body (MGB) and the inferior colliculus (IC) were examined for the presence of mono- and transsynaptically labeled neurons, respectively. The infected neurons were visualized using the specific marker they expressed and by PRV immunolabeling. We confirmed our previous findings namely, that the site of virus injection in the brain cannot be determined based only on labeling of viral proteins. To circumvent this problem and also to identify direct as opposed to transsynaptic connections, the virus was co-injected with monosynaptic retrograde tracers cholera toxin B subunit (CTb) or Fluorogold (FG). Surprisingly, these tracers inhibited viral infection to a degree that it was not observed when the virus was co-injected with a high concentration of monosynaptic tracers. Lowering tracer concentration resulted in an appearance of virus-labeled neurons around CTb- or FG-labeled neurons in the MGB. Very low concentrations of monosynaptic retrograde tracers, that already did not label neurons in the MGB, allowed viral infections throughout the MGB and IC. Since these very low concentrations of tracers were still visible at the injection site, their co-injection is useful in the identification and topographical localization of the injection site following intracranial viral injections. Acknowledgements Grant support: NKTH-OTKA K67646, OTKA CK 80180 and Bolyai Fellowship. Keywords: methods, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Methods Citation: Szabó ÉR, Tombácz D, Boldogkői Z, Palkovits M and Dobolyi Á (2011). Recombinant pseudorabies virus strains as intracranially injected retrograde tracers. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00040 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. Á. Dobolyi, Hungarian Academy of Sciences and Semmelweis University, Department of Anatomy, Histology and Embryology, Budapest, Hungary, dobolyi@ana.sote.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers É. R Szabó D. Tombácz Z. Boldogkői M. Palkovits Á. Dobolyi Google É. R Szabó D. Tombácz Z. Boldogkői M. Palkovits Á. Dobolyi Google Scholar É. R Szabó D. Tombácz Z. Boldogkői M. Palkovits Á. Dobolyi PubMed É. R Szabó D. Tombácz Z. Boldogkői M. Palkovits Á. Dobolyi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.