Recombinant polyepitop-forming chimera protein derived from Borrelia burgdorferi OspC variants induces specific antibodies recognizing wide spectrum of antigenic variants of pathogenic Borreliae.

Abstract

Event Abstract Back to Event Recombinant polyepitop-forming chimera protein derived from Borrelia burgdorferi OspC variants induces specific antibodies recognizing wide spectrum of antigenic variants of pathogenic Borreliae. Michal Krupka1*, Josef Masek2, Romana Cahlikova1, Barbora Knoppova1, Evzen Weigl1, Marek Sebela3, Jaroslav Turanek2 and Milan Raska1 1 Ústav imunologie Lékařské fakulty univerzity Palackého, Czechia 2 Veterinary research institute, Czechia 3 Department of Biochemistry, Faculty of Science, Palacky University in Olomouc, Czechia Lyme disease is the most frequent zoonosis in Europe and in the United States. It may progress into a chronic form and could lead to nervous or cardiovascular system, joints, skin or eyes affections. Outer surface protein C (OspC) is 23 kDa, lipoprotein antigen of spirochete Borrelia burgdorferi species complex - causative agent of Lyme disease. OspC is one of the most promising candidates for vaccine design. Due to high sequence variability of OspC it is not possible to induce protective immunity to broader range of Borrelia strains by single variant of OspC protein. In this study, we created a fusion chimera recombinant protein (chOspC) composed of OspC immunodominant domains from most frequent infective strains of B. burgdorferi. Protein was expressed in E. coli in fusion with His-tag allowing metalloaffinity chromatography purification. Identity of protein was confirmed by MALDI-TOF. Recombinant chOspC was used for the immunization of experimental mice. Hyperimmune sera were tested to evaluate their reactivity with several OspCs using Western blot and human diagnostic line-blot stripes. Mice immunized with B31 variant of OspC served as a control. Sera of chOspC-vaccinated mice reacted specifically and strongly with whole B. burgdorferi bacteria lysates on Western blot forming single band at position corresponding to OspC as well as with all three OspCs from selected pathogenic subspecies on line blot. In contrast, control sera reacted to only subspecies-specific OspC. Described properties are promising for further testing of chOspC protein as potential Lyme disease vaccine component. Acknowledgements Supported by grants LF_2013_13, TA01011165 and GAP304/10/1951. Keywords: Borrelia, OspC vaccine, Lyme Disease, Recombinant Proteins, polyepitope Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Krupka M, Masek J, Cahlikova R, Knoppova B, Weigl E, Sebela M, Turanek J and Raska M (2013). Recombinant polyepitop-forming chimera protein derived from Borrelia burgdorferi OspC variants induces specific antibodies recognizing wide spectrum of antigenic variants of pathogenic Borreliae.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00469 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Michal Krupka, Ústav imunologie Lékařské fakulty univerzity Palackého, Olomouc, Czechia, krupka.olomouc@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Michal Krupka Josef Masek Romana Cahlikova Barbora Knoppova Evzen Weigl Marek Sebela Jaroslav Turanek Milan Raska Google Michal Krupka Josef Masek Romana Cahlikova Barbora Knoppova Evzen Weigl Marek Sebela Jaroslav Turanek Milan Raska Google Scholar Michal Krupka Josef Masek Romana Cahlikova Barbora Knoppova Evzen Weigl Marek Sebela Jaroslav Turanek Milan Raska PubMed Michal Krupka Josef Masek Romana Cahlikova Barbora Knoppova Evzen Weigl Marek Sebela Jaroslav Turanek Milan Raska Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.