Recombinant manganese peroxidase (rMnP) from Pichia pastoris. Part 1: Kraft pulp delignification

Abstract

AbstractManganese peroxidase (MnP) is the main enzyme implicated in the biobleaching of kraft pulps by white-rot fungi. However, potential commercial applications of this enzyme have been limited by its availability in large quantities. Advances have been made to produce high-yield concentrated recombinant MnP (rMnP). The objective of this study was to evaluate the ability of rMnP to delignify and brighten kraft pulps. The rMnP, produced from the yeastPichia pastoris– in high-cell density and in fed-batch fermentations – was found to be effective in lignin removal in both hardwood and softwood unbleached kraft pulps. The rMnP applied at 30 U g-1pulp for 24 h followed by alkali extraction caused significant kappa number reductions for all the pulps tested with different initial lignin contents and structures. Softwood and hardwood pulps showed similar delignification rates during rMnP treatments. Highly delignified pulps with kappa number less than 10 are less susceptible to delignification by rMnP compared with the pulps with higher lignin content. The rMnP-treated pulp was also shown to be more susceptible to subsequent peroxide bleaching compared with the control pulp. More than 60% of the kappa number reduction was achieved by sequential rMnP treatments combined with alkaline extraction. Sequential treatment with xylanase and rMnP also resulted in more extensive delignification than in each enzyme treatment alone or in the case of simultaneous application of the enzymes.