Abstract
A family 26, glycoside hydrolase (GH26) named lichenase of the molecular size 30 kDa fromClostridium thermocellum previously cloned into an expression vector pET21a was over-expressed inEscherichia coli BL-21 cells and was purified to homogeneity by a single step purification method using immobilised metal ion affinity-chromatography. The purified enzyme showed no activity towards soluble substrates such as carboxymethyl cellulose, hydroxymethyl cellulose, laminarin, galactomannan or glucomannan. However, unlike other members of the family GH26, the enzyme lichenase showed high activity towards lichenan and β-glucan and was highly specific endo acting β-1,3-1,4-glucanase. Quantitative affinity-gel electrophoresis on native-PAGE showed that lichenase binds only glucomannan (with aKa of 41.3 C−1) and not lichenan or β-glucan.
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