Abstract
We expressed the extracellular domain (20–408 aa, (T)) of human TSH receptor (TSHR) in E. coli to detect TSHR autoantibodies (TRAb) and, moreover, we expressed the two portions (20–218 aa (5′) and 217–408 aa (3′)) of the extracellular domain thought to distinguish thyroid stimulating antibodies (TSAb) from blocking antibodies (TSBAb), using pGEX.3X as the expression vector. Using Western blotting analysis of the sera from patients with autoimmune thyroid disease, sera from Graves' patients and patients with idiopathic myxedema who had TSBAb reacted with the fusion protein (T), but none of the control sera reacted with it. We further evaluated whether or not the positive sera for T recognized fusion proteins (5′) or (3′). The sera from Graves' patients reacted with both fusion proteins (5′) and (3′). The sera from patients with idiopathic myxedema did not react with either of fusion proteins (5′) or (3′). These findings suggest that these recombinant TSHR proteins could be used as antigens to detect TRAb, and differentiate TSBAb from patients with idiopathic myxedema.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
