Abstract
Background Bone Morphogenetic Proteins (BMPs) are multifunctional, secreted cytokines belonging to the TGF-b superfamily. These proteins act as a disulfide-linked homodimer, being potent regulators of bone and cartilage formation and repair, cell proliferation in embryonic development and adult bone homeostasis. BMPs are promising molecules in periodontal regeneration to treat physiopathological bone loss and non-union fractures and in oral surgery, and to accelerate and increase osseointegration. BMPs are dimeric molecules displaying sites for Nand O-glycosylation, which increases the stability and half-life of the protein in the body, in addition to determining the specificity of receptor coupling. BMP-2 induces cartilage and bone formation. BMP4 has also been shown to play a role in triggering osteoblastic differentiation of mesenchymal stem cells, through activation of osteoblastic related genes. In order to ensure proper glycosylation and conformational folding and to prevent immunogenicity, we elected a mammalian cell expression system to produce these BMPs aiming at bone regeneration, stem cell proliferation and differentiation and their application in human and veterinary cell therapy.
Highlights
Bone Morphogenetic Proteins (BMPs) are multifunctional, secreted cytokines belonging to the TGF-b superfamily
BMPs 2 and 4 cDNAs were amplified from an in-house constructed cDNA Bank and cloned into the pGEM®-TEasy vector
HEK293 cells were co-transfected with a lentiviral plasmid containing both BMP 2 or 4 and eGFP cDNAs, by co-transfection, at a 40:1 ratio, with a Hygror vector for clone selection
Summary
Bone Morphogenetic Proteins (BMPs) are multifunctional, secreted cytokines belonging to the TGF-b superfamily. Methods BMPs 2 and 4 cDNAs were amplified from an in-house constructed cDNA Bank and cloned into the pGEM®-TEasy vector. Upon DNA sequencing, the BMP 2 and 4 inserts were transferred to a lentiviral expression vector. HEK293 cells were co-transfected with a lentiviral plasmid containing both BMP 2 or 4 and eGFP cDNAs, by co-transfection, at a 40:1 ratio, with a Hygror vector for clone selection.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
