Recloned colonies positive for T-cell-associated antigens derived from mixed hemopoietic colonies (CFU-GEMM)1.

Abstract

AbstractHuman pluripotent hemopoietic progenitors (CFU-GEMM) can be identified by their ability to form colonies in culture that contain granulocytes, erythroblasts, macrophages, and megakaryocytes. Forty-six individual mixed colonies from four individuals were prepared by cytocentrifugation and examined for cells positive for human T-cell markers using the monoclonal antibodies E 2-22 and OKT 3 and fluorescently labeled goat F(ab')2 anti-mouse IgG antibody. Sixteen primary mixed colonies were found to be positive for the monoclonal antibodies E 2-22 and OKT 3. Seventy-nine primary mixed colonies derived from these four individuals were aspirated, dispersed, and then recloned. Twenty-three primary mixed colonies formed 97 secondary hemopoietic colonies. All cells in 50 recloned hemopoietic colonies were positive for the antibodies directed against T cells.