Abstract
Chitinous materials from marine byproducts elicit great interest among biotechnologists for their potential biomedical or agricultural applications. In this study, four kinds of marine chitinous materials (squid pens, shrimp heads, demineralized shrimp shells, and demineralized crab shells) were used to screen the best source for producing chitosanase by Paenibacillus macerans TKU029. Among them, the chitosanase activity was found to be highest in the culture using the medium containing squid pens as the sole carbon/nitrogen (C/N) source. A chitosanase which showed molecular weights at 63 kDa was isolated from P. macerans cultured on a squid pens medium. The purified TKU029 chitosanase exhibited optimum activity at 60 °C and pH 7, and was stable at temperatures under 50 °C and pH 3-8. An analysis by MALDI-TOF MS revealed that the chitosan oligosaccharides (COS) obtained from the hydrolysis of water-soluble chitosan by TKU029 crude enzyme showed various degrees of polymerization (DP), varying from 3–6. The obtained COS enhanced the growth of four lactic acid bacteria strains but exhibited no effect on the growth of E. coli. By specialized growth enhancing effects, the COS produced from hydrolyzing water soluble chitosan with TKU029 chitinolytic enzymes could have potential for use in medicine or nutraceuticals.
Highlights
Chitosan is a polysaccharide consisting of 1,4-ß-linked D-glucosamine residues, partially substituted with N-acetyl group
Four kinds of the marine byproducts, i.e., squid pen powder (SPP), demineralized (SHP),crab were used as the sole C/N source to explore the production of chitosanase by P. macerans shell powder, demineralized shrimp shell powder, and shrimp head powder
Chitinous materials have been proposed as an important factor for producing Chitinous materials have been proposed as an important factor for producing chitinase/chitosanase by fermentation [22]
Summary
Chitosan is a polysaccharide consisting of 1,4-ß-linked D-glucosamine residues, partially substituted with N-acetyl group. Growing on squid pens, shrimp shells, or crab shells crab shells or squid pens have been used as the sole C/N source for comparing the activities have been reported; there have been few reports about chitinase production of Paenibacillus of chitinase, chitosanases, and α-glucosidase inhibitors by demineralized. Four kinds of the marine byproducts, i.e., squid pen powder (SPP), demineralized (SHP),crab were used as the sole C/N source to explore the production of chitosanase by P. macerans shell powder (deCSP), demineralized shrimp shell powder (deSSP), and shrimp head powder. The oligomers obtainedand by hydrolyzing water-soluble chitosan with TKU029 chitosanase were analyzed by MALDI-TOF-MS, and their enhancing effect on the growth of lactic acid bacteria was estimated
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