Reciprocal inhibiting interactive mechanism between the estrogen receptor and aryl hydrocarbon receptor signaling pathways in goldfish (Carassius auratus) exposed to 17β-estradiol and benzo[a]pyrene

Abstract

In the aquatic environment, both the estrogen receptor (ER) and aryl hydrocarbon receptor (AhR) responses are established biomarkers for assessing exposure to pollutants. These receptor responses can also be affected by the presence of other classes of pollutants and may result in misinterpretation of existing pollution. In this study, we investigated the interaction between ER–vitellogenin (VTG) and AhR–cytochrome P450 1A (CYP1A) signaling pathways in goldfish (Carassius auratus) after 10days exposure to pollutants. 17β-Estradiol (E2) and benzo[a]pyrene (BaP) were selected as the ER and AhR agonists, respectively. The messenger RNA (mRNA) expression of ER–VTG and AhR–CYP1A in liver was determined using quantitative real-time polymerase chain reaction (QRT–PCR). VTG, endogenous E2 and 7-ethoxyresorufin-O-deethylase (EROD) were also studied. Exposure to E2 and BaP alone significantly induced the gene expression of ERα–VTG and AhR2–CYP1A, respectively. Moreover, the obvious expression of related proteins was also observed. However, these inductions were significantly reduced after combined exposure to E2 and lower concentrations of BaP (20 and 50μg/L), indicative of a reciprocal inhibiting ER–AhR interaction. However, high concentrations (100μg/L) of BaP did not affect the E2-induced gene expression. Changes in VTG protein were in accordance with the expression of VTG mRNA, and more VTG protein was observed in liver than in serum. The induced endogenous E2 levels were suppressed by the presence of BaP. While the gene expression of CYP1A showed a concentration-dependent increase, EROD induction exhibited a bell-shaped concentration-response curve. Taken together, these results demonstrate a reciprocal inhibiting mode of ER–AhR interactions and may lead to a possible underestimation of actual exposure.