Abstract
P969 Introduction: According to some reports, immature donor derived myeloid (DC) prolong heart allograft survival. We reported previously that depletion of mature DC in donor heart and replacing them with recipient bone marrow derived DC did not prolong survival of heart allograft but to the contrary induced rejection of allogeneic and even syngeneic grafts. In this study we investigated influence of recipient immature BM-derived DC on heart allograft survival. Material and methods: Depopulation of prospective recipient was achieved by total body irradiation. Next, repopulation by syngeneic immature DC (BN to BN) from vascularised BM transplant followed. Three days after repopulation from transplanted BM, rats received allogeneic heart transplant from LEW rats. We compared heart allograft survival in recipients repopulated with non-repopulated (irradiated), non-depopulated (naive) recipients, and repopulated with syngeneic granulocytes. Immunohistological study of allograft recipients graft, spleen, lymph nodes and BM using mAb against OX 27 (specific for donor only) and OX6, OX62, ED1, W3/13 was performed after depopulation, repopulation and rejection. Double staining by OX27 and the above listed antibodies was carried out using flow cytometry. A low level of OX27/ OX6 and OX27/OX62 expression (fluorescence) reflected immaturity of DC. Results: Allograft survival in recipients repopulated with immature BM-derived DC was - 14±2 days, in non-repopulated (irradiated) recipient - 13±1 days, in non-depopulated (naive) recipient - 7±1 days, in irradiated and repopulated with syngeneic granulocytes - 15±2 days. There was low antigen expression of OX27/OX6 and OX27/OX62 antigens in repopulated hearts before transplantation and similar density of infiltrating OX6+ and OX27+ cells in rejecting allografts. Conclusion: In this model with donor heart repopulated by immature recipient DC no biologically significant prolongation of allograft survival was observed.
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