Abstract
We examined the effects of peptide YY (PYY), neuropeptide Y (NPY), and their analogues on dispersed pancreatic acini. Binding of [ 125I]PYY to acini was saturable, reversible, and specific, and PYY binding was best fit with a two-site model. The relative potencies for inhibiting [ 125I]PYY binding were PYY ≥ NPY > NPY(13–36) . There was no inhibition of binding with [Leu 31,Pro 34]NPY, PYX-2, or pancreatic polypeptide. Both PYY and NPY (0.1 μ M) inhibited amylase release stimulated by vasoactive intestinal polypeptide (VIP) (0.3 n M) and forskolin (1 μ M) by about 30%, but not that stimulated by cholecystokinin-8 or bombesin. The relative potencies for inhibiting VIP-stimulated amylase release were PYY ≥ NPY > NPY(13–36) , the same as those for inhibiting VIP-stimulated cAMP increase in acini. No inhibition was detected with [Leu 31,Pro 34]NPY. This work demonstrates Y 2 receptors on guinea pig pancreatic acini mediating inhibitory actions of PYY and NPY on pancreatic enzyme secretion.
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