Abstract
A HT 29 cell line derived from human colonic carcinoma was shown to express the intrinsic factor receptor, with about 5000 binding sites per cell and an association constant of 20 × 10 9 1/mol at pH 7.4 and 4°C. The number of binding sites increased dramatically between 7 and 10 days of culture time. Endocytosis of the intrinsic factor-cobalamin-receptor complex was inhibited by two ways: at 4°C and at 37°C by incubating the cells with vinblastine, monensin and chloroquine. The plasma membrane receptor was cross-linked to [ 57Co]cobalamin-intrinsic factor and solubilized with Triton X-100. The cross-linked complex had a relative molecular mass of 330 kDa in native PAGE.
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