Abstract
Abstract: Alkaloids provide significant health benefits in moderation, but excessive levels can pose health hazards. They play an important role in the creation of numerous pharmacological medications, acting as potent antispasmodics, analgesics, and even anti-cancer medicines. A detailed review of sensitive and accurate alkaloid analytical techniques can be used as a guide for future analyses of alkaloids in pertinent research. The main aim of this manuscript is to review the literature on the detection and separation of alkaloids by using various methods like analytical, bioanalytical, and electrochemical techniques, published during 2018-2023. An in-depth review of the literature was carried out using a variety of databases, including Web of Knowledge, PubMed, and Google Scholar. Consulting relevant published materials, including books, was another aspect of this research. The keywords used in the search were alkaloids, analytical techniques, bio-analytical techniques, electrochemical techniques, and biosensors. These were carefully examined in more detail, and significant data and findings were collected and presented using tables. The publication highlights the significance of advanced chromatographic techniques in the separation and isolation of alkaloids. It discusses several analytical, bio-analytical, and electrochemical analytical techniques, which include sensors and biosensors, and adds to the extensive review of alkaloid detection techniques. Recent advancements and methodologies for improving the knowledge of the detection and separation of alkaloids are presented in this article, which is beneficial for the researcher involved in developing analytical methods for alkaloid detection. Current efforts and advanced analytical approaches for alkaloid detection are given in this manuscript, which is crucial in favor of improving the health and wellness of society.
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