Abstract
Paradoxical Concanavalin A staining(PCS) is a histochemical method for identifying class III mucin or gastric gland mucous cell−type mucin. This mucin is largely found in mucous neck cells and pyloric gland cells of the gastric mucosa as well as Brunner’s glands of the duodenum. Recently, we have established that the carbohydrate moiety identified by PCS is GlcNAcα 1→4Galβ→R by cloning a glycosyltransferase, α1, 4−N−acetylglucosaminyltransfearase(α4GnT). In this review, we have focused on our cloning strategy of the glycosyltransferase, α4GnT and determination of carbohydrate moiety responsible for class III Con A reactivity.
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