Abstract
Legumes, as protein-rich crops, are widely used for human food, animal feed and vegetable oil production. Over the past decade, two legume species, Medicago truncatula and Lotus japonicus, have been adopted as model legumes for genomics and physiological studies. The tobacco transposable element, Tnt1, is a powerful tool for insertional mutagenesis and gene inactivation in plants. A large collection of Tnt1-tagged lines of M. truncatula cv. Jemalong was generated during the course of the project ‘GLIP’: Grain Legumes Integrated Project, funded by the European Union (www.eugrainlegumes.org). In the project ‘IFCOSMO’: Integrated Functional and COmparative genomics Studies on the MOdel Legumes Medicago truncatula and Lotus japonicus, supported by a grant from the Ministry of Education, Youth and Science, Bulgaria, these lines are used for development of functional genomics platform of legumes in Bulgaria. This review presents recent advances in the evaluation of the M. truncatula Tnt1 mutant collection and outlines the steps that are taken in using the Tnt1-tagging for generation of a mutant collection of the second model legume L. japonicus. Both collections will provide a number of legume-specific mutants and serve as a resource for functional and comparative genomics research on legumes. Genomics technologies are expected to advance genetics and breeding of important legume crops (pea, faba bean, alfalfa and clover) in Bulgaria and worldwide.
Highlights
Plant genomics research started with a complete genome sequencing of the model plant Arabidopsis thaliana in December 2000 [1]
This review presents recent advances in the evaluation of the M. truncatula Tnt1 mutant collection and outlines the steps that are taken in using the Tnt1-tagging for generation of a mutant collection of the second model legume L. japonicus
The ‘Medicago truncatula’ Sequencing Consortium is an international partnership of research laboratories, which is decoding the genome sequence of M. truncatula
Summary
Plant genomics research started with a complete genome sequencing of the model plant Arabidopsis thaliana in December 2000 [1]. In order to generate a mutant collection of the second model legume L. japonicus, particular attention is given to the use of the Tnt1mediated mutagenesis for establishment of starter lines for this model legume Both collections will provide a number of legume-specific mutants and will serve as a base for advanced functional and comparative genomics research on the legumes. Based upon successful activity of the worldwide Medicago consortium, we propose an idea for initiation and development of a Tnt mutant collection for the second model legume L. japonicus. This collection will provide a number of legume-specific mutants and will serve as a resource for advanced functional genomics research of L. japonicus. The prospect of integrating genome information from both model legumes to achieve a platform for comparative genomics will provide novel insights into the organization and evolution of legumes, as well as the similarities and differences with genomes of other plant families
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