Recent Perspective of Next Generation Sequencing: Applications in Molecular Plant Biology and Crop Improvement

Abstract

Nearly after three decades of Sanger sequencing method dominating the field of nucleotide sequencing, the new age of novel sequencing techniques commenced with 454-pyrosequencing in the year 2005 and thus started the era of next generation sequencing techniques. Since then many other novel sequencing techniques with increased accuracy, simplicity and cost-effectiveness have come up and called next to next generation sequencing techniques or third generation sequencing techniques. The scientific knowledge generated from next generation sequencing techniques has transformed the field of structural and functional genomic studies in different crop plants. In this review, various next generation sequencing techniques are described, with their applications and future prospective.