Abstract
A rapid serologic test using a fluorescein isothiocyanate-conjugated (FITC) anti-IgG serum has been evaluated. Human serum reacted with antigen (coccidioidin)-coated millipore disks provided the matrix for testing. Values were obtained from fluorometer readings. Results of testing more than 100 sera have indicated a close correlation with complement-fixation and agar gel precipitin inhibition (AGP1) titers. Sera tested were positive by either slide latex agglutination (SLA) or immunodiffusion (ID) tests, or both. The soluble antigen fluorescent antibody (SAFA) test appears to be worthy of consideration for use in laboratories not equipped to perform complement-fixation testing. As with the AGPI test, the SAFA procedure can be used with single serum specimens, with consequent earlier reporting of results. Preliminary studies suggest that varying the final FITC reagent might allow evaluation of the contribution of IgM and IgA to positive serology.
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