Abstract
Recent advancements in analytical methods for vitamin D and its metabolites have substantially enhanced our capacity to precisely determine and quantify these substances in a wide range of sources, such as biological fluids, fungus, natural and fortified foods. This study focuses on the latest advancements in sample preparation procedures, including solid-phase extraction and environmentally friendly extraction methods. These approaches aim to enhance efficiency and minimize the use of solvents. In addition, we explore the growing popularity of chromatographic methods, specifically LC-MS/MS and developing supercritical fluid chromatography (SFC), which provide improved sensitivity, selectivity, and faster analytical times for comprehensive vitamin D profiling. These developments are crucial for overcoming the challenges presented by low concentrations and complex matrices in the investigation of vitamin D.
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