Abstract
In the aspect of biomacromolecule imaging, high-speed atomic force microscopy (HS-AFM) has many wonderful characteristics, such as rapid imaging and real-time visualisation of the structure and dynamic behaviour of biological macromolecules, which is unparalleled by other technologies. In this review, state-of-the-art achievements on HS-AFM in molecular biology are summarised. Firstly, biomacromolecule characteristics determination and nanostructure recombination using HS-AFM are introduced, which confirm that HS-AFM is such a mature technology that realises high-speed imaging. Then, some improvements including the cantilever of HS-AFM, mechanical scanner, position sensor, and closed-loop control algorithm are reviewed to obtain higher resolution images. Finally, future development directions that could enhance the performance of HS-AFM are discussed.
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