Abstract
The design of a gene delivery system requires a complete understanding of the interaction between targeting cell and delivery systems. The poly(cystaminebis- (acrylamide)-diaminohexane) (poly(CBA-DAH)) has been confirmed as a bio-reducible efficient delivery carrier among the various systems. Poly(ethylenimine) (PEI, 1.8?kDa) was conjugated to poly(CBA-DAH) via a disulfide bond. The PEI conjugated poly(CBA-DAH) (PCDP) was able to bind with pDNA at a very low molecular weight ratio and form the polyplexes with nano-size and positive surface charge. To confirm the transfection efficiency, the plasmid DNA encodes with the luciferase reporter gene and green fluorescent protein reporter gene have made to use a bio-reducible efficient gene delivery. The PCDP polyplexes show 10 times higher gene transfection efficiency than Lipofectamine® polyplexes in bio-mimic in vivo condition. It has been reviewed that the bio-reducible PEI(1.8kDa) conjugated poly(CBA-DAH) is finally concluded as an efficient gene delivery carrier.
Highlights
Gene therapy has used in the administration of genetic materials such as plasmid deoxyribonucleic acid (pDNA), messenger ribonucleic acid (mRNA), and small interfering ribonucleic acid (siRNA) into specific host cells for the treatment of acquired disorders[1,2,3]
Gene therapy has used in the administration of genetic materials such as plasmid deoxyribonucleic acid, messenger ribonucleic acid, and small interfering ribonucleic acid into specific host cells for the treatment of acquired disorders[1,2,3]
The gene delivery systems consist of three components: i) a plasmid-based gene expression system that controls the function of a gene within the targeting cell, ii) a gene that encodes a specific therapeutic protein, and iii) a gene delivery system that controls the delivery of the gene expression plasmid to specific location within the body[4, 5]
Summary
Gene therapy has used in the administration of genetic materials such as plasmid deoxyribonucleic acid (pDNA), messenger ribonucleic acid (mRNA), and small interfering ribonucleic acid (siRNA) into specific host cells for the treatment of acquired disorders[1,2,3]. The PEI conjugated poly(CBA-DAH) (PCDP) was able to bind with pDNA at a very low molecular weight ratio and form the polyplexes with nano-size and positive surface charge. It has been reviewed that the bio-reducible PEI(1.8kDa) conjugated poly(CBA-DAH) is concluded as an efficient gene delivery carrier. The design of PEI-conjugated bio-reducible polymer for efficient gene delivery has investigated to confirm the successful vectors and polyplexes formation with pDNA.
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