Abstract
DNA biosensors are realised immobilising a DNA structure on a suitable transducer to obtain selective information. In this paper we show how the determination of low-molecular weight compounds with affinity for DNA was measured by their effect on the oxidation signal of the guanine peak of calf thymus DNA immobilised on the electrode sensor and investigated by chronopotentiometric analysis. The DNA biosensor is able to detect known intercalating and groove binding compounds. Applicability to river water samples was demostrated. Moreover, a piezoelectric sensor coupled to a short oligonucleotide can be used as detector of the hybridisation reaction. We show as a model the detection of a specific mutation in apolipoprotein E (ApoE) gene. Biotinylated 23-mer probes were immobilised on the streptavidin coated gold surface of a quartz crystal; the protein was covalently bound to the thiol/dextran modified gold surface. The device was able to distinguish different synthetic oligonucleotides. The hybridisation reaction was also performed using real samples of DNA extracted from human blood and amplified by Polymerase chain reaction PCR. The extension of such procedure to samples of environmental interest is discussed.
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