Recent advances in tools and technologies for monitoring and controlling ovarian activity in marsupials

Abstract

Components of assisted reproduction technologies (ART), such as sperm cryopreservation, artificial insemination, superovulation and pouch young surrogacy, have been developed for a range of Australian and American marsupials. However, methods to effectively control ovarian function, arguably the key limiting factors in applying and integrating ART as a practical tool in conservation management, remain poorly developed. This is largely due to unique characteristics of the marsupial corpus luteum and its failure to respond to agents used to synchronize ovarian function in eutherian mammals. This paper presents an overview of relevant aspects of marsupial reproductive biology across marsupial taxonomic groups including information on the long-established technique of removal of suckling young to activate ovarian cycles. Ovarian monitoring tools for marsupials are reviewed and their usefulness for ART assessed (laparotomy, hormone cycling, vaginal cytology, laparoscopy and ultrasonography). We also discuss promising recent work examining the potential of manipulating hypothalamic-pituitary function using GnRH agonists and antagonists as the basis of ovarian control (female synchronization) strategies.