Recent Advances in the Phylogenetic Analysis to Study Rumen Microbiome

Abstract

Background: Recent rumen microbiome studies are progressive due to the advent of nextgeneration sequencing technologies, computational models, and gene referencing databases. Rumen metagenomics enables the linking of the genetic structure and composition of the rumen microbial community to the functional role it plays in the ecosystem. Systematic investigations of the rumen microbiome, including its composition in cattle, have revealed the importance of microbiota in rumen functions. Various research studies have identified different types of microbiome species that reside within the rumen and their relationships, leading to a greater understanding of their functional contribution. Objective: The objective of this scoping review was to highlight the role of the phylogenetic and functional composition of the microbiome in cattle functions. It is driven by a natural assumption that closely related microbial genes/operational taxonomical units (OTUs)/amplicon sequence variants (ASVs) by phylogeny are highly correlated and tend to have similar functional traits. Methods: PRISMA approach has been used to conduct the current scoping review providing state-ofthe- art studies for a comprehensive understanding of microbial genes’ phylogeny in the rumen microbiome and their functional capacity. Results: 44 studies have been included in the review, which has facilitated phylogenetic advancement in studying important cattle functions and identifying key microbiota. Microbial genes and their interrelations have the potential to accurately predict the phenotypes linked to ruminants, such as feed efficiency, milk production, and high/low methane emissions. In this review, a variety of cattle have been considered, ranging from cows, buffaloes, lambs, Angus Bulls, etc. Also, results from the reviewed literature indicate that metabolic pathways in microbiome genomic groupings result in better carbon channeling, thereby affecting methane production by ruminants. Conclusion: The mechanistic understanding of the phylogeny of the rumen microbiome could lead to a better understanding of ruminant functions. The composition of the rumen microbiome is crucial for the understanding of dynamics within the rumen environment. The integration of biological domain knowledge with functional gene activity, metabolic pathways, and rumen metabolites could lead to a better understanding of the rumen system.