Abstract
Cell sheet engineering has evolved rapidly in recent years as a new approach for cell-based therapy. Cell sheet harvest technology is important for producing viable, transplantable cell sheets and applying them to tissue engineering. To date, most cell sheet studies use thermo-responsive systems to detach cell sheets. However, other approaches have been reported. This review provides the progress in cell sheet detachment techniques, particularly reactive oxygen species (ROS)-responsive strategies. Therefore, we present a comprehensive introduction to ROS, their application in regenerative medicine, and considerations on how to use ROS in cell detachment. The review also discusses current limitations and challenges for clarifying the mechanism of the ROS-responsive cell sheet detachment.
Highlights
The development of tissue engineering technology has shown considerable potential in the field of regenerative medicine [1]
The temperature-responsive surfaces allow harvesting cultured cells without the use of proteolytic enzymes, such as trypsin or dispase, which can lead to cell membrane and surface antigen damage and loss of differentiated phenotypes [6,7]
This review provides an overview of current techniques for creating cell sheets using various systems, reaction oxygen species (ROS)-responsive systems
Summary
The development of tissue engineering technology has shown considerable potential in the field of regenerative medicine [1]. It has already demonstrated clinical results that can be transplanted into various animal models to restore or enhance original tissue functions [2,3,4,5] These results indicated the emergence of better regenerative therapies than cell suspension injection. Cell sheets with ECM can be transplanted directly to the target site or stacked to produce structures similar to three-dimensional (3D) tissue [8]. This approach may address the limitations of existing regenerative therapies, such as cell injection and tissue reconstruction using biodegradable scaffolds. Sci. 2019, 20, x FOR PEER REVIEW for creating cell sheets using various systems, reaction oxygen species (ROS)-responsive systems
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