Abstract
Messenger RNA (mRNA) translation is a central step of gene expression. Translational regulation plays essential roles in various biological processes. Ribosome profiling is a powerful technique to study genome-wide translational regulation at a single-codon resolution. Based on conventional ribosome profiling procedures, many new experimental methods have recently been developed to investigate the biology of mRNA translation from different aspects. These technical advances have significantly advanced our understanding of translational regulation at genomic scales from the following perspectives: (1) identification of translational initiation sites; (2) characterization of the dynamics of the complete translation cycle; (3) profiling of active ribosomes; (4) tissue-specific translational regulation; (5) translational regulation in subcellular locations; (6) detection of ribosome traffic jams; (7) plastid translatomes; and (8) ribosome heterogeneity and translational preference. Here, we summarize the key differences between these new techniques and the conventional ribosome profiling approach. We also review the computational methods designed to analyze ribosome profiling data.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
