Recent advances in phytochemistry, vol. 31, functionality of food phytochemicals : edited by T. Johns, J.T. Romeo, Plenum Press, New York, 1997, 273 p., $95.00

Abstract

Heretofore, transgenesis in the parasitic nematode genus Strongyloides has relied on microinjecting transgene constructs into gonadal syncytia of free-living females. We now report transgenesis in Strongyloides stercoralis by microinjecting constructs into the syncytial testes of free-living males. Crosses of individual males microinjected with a construct encoding GFP with cohorts of 12 non-injected females produced a mean of 7.28 ± 2.09 transgenic progeny. Progeny of males and females microinjected with distinct reporter constructs comprised 2.6% ± 0.7% of individuals expressing both paternal and maternal transgenes. Implications of this finding for deployment of CRISPR/Cas9 mutagenesis in Strongyloides spp. are discussed.