Abstract
Enzymatic oxidation reactions may offer ‘greener’ alternatives to chemical oxidation processes, which have typically involved toxic metal catalysts, generation of toxic byproducts or poor atom economy. Oxidases function at ambient temperature and pressure with no external cofactor recycling systems required. This review examines recent progress in oxidases, including enzyme discovery and engineering, and the application of these biocatalysts in cascade reactions. Process requirements including reactor design, oxygen supply and use of non-aqueous solvents are explored for the utilisation of oxidases on industrial scale.
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