Abstract
Recent advances in fluorescence imaging techniques and super-resolution microscopy have extended the applications of fluorescent probes in studying various cellular processes at the molecular level. Specifically, organelle-targeted probes have been commonly used to detect cellular metabolites and transient chemical messengers with high precision and have become invaluable tools to study biochemical pathways. Moreover, several recent studies reported various labeling strategies and novel chemical scaffolds to enhance target specificity and responsiveness. In this review, we will survey the most recent reports of organelle-targeted fluorescent probes and assess their general strategies and structural features on the basis of their target organelles. We will discuss the advantages of the currently used probes and the potential challenges in their application as well as future directions.
Highlights
Fluorescent chemical probes have been extensively used to study biochemical events within live cells
We provide an overview of the most prominent applications and advantages of organelle-targeted probes and discuss potential challenges and future directions
Lysosomal H+ and other ions such as Ca2+, Fe2+, Zn2+, and Cl− are the key regulators of lysosomal function; impaired ion homeostasis can lead to defects in lysosomal trafficking and storage, which are associated with neurodegenerative diseases and metabolic disorders [2,3]
Summary
Fluorescent chemical probes have been extensively used to study biochemical events within live cells. The probe had a strong emission at 505 nm, which decreased upon reaction with ClO− because of the oxidation of the thioester; the fluorescence signals with decreased intensity were recovered by the subsequent addition of GSH, suggesting that the probe can be used to monitor the intracellular HClO/GSH redox cycle Another strategy introduced by Ren et al demonstrated that a photocaged probe containing a morpholine and a dibenzoylhydrazine group (5) can be effectively localized inside lysosomes and can release the reactive probe upon UV light illumination [12]. The co-treatment of the modified NLS (pep6) and NP-1-loaded cells showed a much improved nuclear uptake compared to that of their previously reported probe; it took 18 h for the optimal uptake and click reaction, which may not be suitable for real-time tracking of nuclear H2O2 in live cells under various physiological conditions In this example, stepwise in situ labeling targeting the nucleus seems feasible, it needs to be improved for practical use
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
