Abstract

Phytopathogenic fungal species can cause enormous losses in quantity and quality of crop yields and this is a major economic issue in the global agricultural sector. Precise and rapid detection and identification of plant infecting fungi are essential to facilitate effective management of disease. DNA-based methods have become popular methods for accurate plant disease diagnostics. Recent developments in standard and variant polymerase chain reaction (PCR) assays including nested, multiplex, quantitative, bio and magnetic-capture hybridization PCR techniques, post and isothermal amplification methods, DNA and RNA based probe development, and next-generation sequencing provide novel tools in molecular diagnostics in fungal detection and differentiation fields. These molecular based detection techniques are effective in detecting symptomatic and asymptomatic diseases of both culturable and unculturable fungal pathogens in sole and co-infections. Even though the molecular diagnostic approaches have expanded substantially in the recent past, there is a long way to go in the development and application of molecular diagnostics in plant diseases. Molecular techniques used in plant disease diagnostics need to be more reliable, faster, and easier than conventional methods. Now the challenges are with scientists to develop practical techniques to be used for molecular diagnostics of plant diseases. Recent advancement in the improvement and application of molecular methods for diagnosing the widespread and emerging plant pathogenic fungi are discussed in this review.

Highlights

  • Fungal plant pathogens are among the foremost biotic factors that cause devastating disease in crops (Doehlemann et al, 2017)

  • This review aims to summarize various molecular techniques in fungal plant pathogen diagnosis along with their advantages and drawbacks

  • The results revealed that there was a dramatic shift in the Puccinia striiformis f. sp. tritici (PST) population in the UK, likely due to a current introduction of a different set of emerging and exotic PST lineages (Hubbard et al, 2015)

Read more

Summary

Frontiers in Cellular and Infection Microbiology

Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review. Recent developments in standard and variant polymerase chain reaction (PCR) assays including nested, multiplex, quantitative, bio and magnetic-capture hybridization PCR techniques, post and isothermal amplification methods, DNA and RNA based probe development, and next-generation sequencing provide novel tools in molecular diagnostics in fungal detection and differentiation fields. These molecular based detection techniques are effective in detecting symptomatic and asymptomatic diseases of both culturable and unculturable fungal pathogens in sole and co-infections.

INTRODUCTION
MOLECULAR TOOLS FOR DETECTION OF FUNGI
Wheat Strawberry
Citrus black spot
Isothermal Amplification Based Methods
Potato late blight
Post Amplification Techniques
DNA or RNA Probe Based Assays
CONCLUSION
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call