Abstract
Microfluidics-based liquid chromatography is based on the miniaturization of the different types of liquid chromatography (LC) systems (e.g., affinity, adsorption, size exclusion, ion exchange) on a microchip to perform on-chip separation of different types of analytes. On-chip chromatography finds applications in genomics, proteomics, biomarker discovery, and environmental analysis. Microfluidics-based chromatography has good reproducibility and small sample consumption. However, the on-chip chromatography fabrication techniques are often more challenging to perform than conventional LC column preparation. Different research groups have attempted to develop different techniques to fabricate microfluidics-based LC systems. In this review, we will summarize the recent advances in microfluidics-based chromatography.
Highlights
Micro total analysis systems have a variety of advantages over large systems, including less sample consumption, rapid analysis speed, and good portability
On-chip liquid chromatography (LC) systems can be readily coupled with different types of detection systems, such as mass spectrometry (MS) [7,8], fluorescent microscopy [9], and conductivity detectors [10]
LC columns based on their sepaand discuss the recent advances of microfluidic on their separation ration mechanisms
Summary
Micro total analysis systems (μTAS) have a variety of advantages over large systems, including less sample consumption, rapid analysis speed, and good portability. The development of different on-chip LC systems is attracting more interest than ever before. Several different types of columns can be integrated on the chip to realize online desalting and multidimensional separation [12]. A microchip with antibacteria/virus antibody modification can be applied for Separations 2021, 8, 3. A microchip with antibacteria/virus antibody modification can be applied for capturing and recognizing bacteria and in biofluids [13]. [16,17],size-exclusion-based size-exclusion-based [18], hydrodyclasses:affinity-based, affinity-based, adsorption-based [18], hydrodynamicnamic-based separation, and ion-exchange-based columns. This review, we summarize will sumbased separation, and ion-exchange-based columns. LC columns based on their sepaand discuss the recent advances of microfluidic on their separation ration mechanisms.
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