Abstract
Recently, mass spectrometric related techniques have been widely applied for the identification and quantification of neurochemicals and their metabolites in biofluids. This article presents an overview of mass spectrometric techniques applied in the detection of neurological substances and their metabolites from biological samples. In addition, the advances of chromatographic methods (LC, GC and CE) coupled with mass spectrometric techniques for analysis of neurochemicals in pharmaceutical and biological samples are also discussed.
Highlights
Neuroscience is the subject to study the chemical composition and processes of the nervous system, which includes the brain, the spinal cord and the nerves and the effects of chemicals on them
We introduce the recent advances in mass spectrometric methods for the identification of neurological substances from the following techniques: (i) extraction methods coupled with MS; (ii) chromatographic techniques coupled with MS; (iii) direct mass spectrometric tools for the identification of neurological chemicals in biofluids
Mass spectrometry has matured as a versatile, high-performance technique for sensitive analysis of neurological substances in biological and drug-abuse samples
Summary
Neuroscience is the subject to study the chemical composition and processes of the nervous system, which includes the brain, the spinal cord and the nerves and the effects of chemicals on them. Ghosh’s group developed a rapid and sensitive method via SPE coupled with LC-ESI-MS/MS for simultaneous determination of ENP and its metabolite (ENPT) in human plasma [31]. The mass transitions: m/z 146 87 for ACh and m/z 15587 for the internal standard ACh-D9 were confirmed by low-energy ESI-MS/MS in the positive ion mode with MRM, Keski-Rahkonen and co-workers developed a rapid, simple and sensitive LC-APCI-MS/MS method for determination of ACh in microdialysis samples of rat brains [35].
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