Abstract
Pathogenic bacterial contamination greatly threats human health and safety. Rapidly biosensing pathogens in the early stage of infection would be helpful to choose the correct drug treatment, prevent transmission of pathogens, as well as decrease mortality and economic losses. Traditional techniques, such as polymerase chain reaction and enzyme-linked immunosorbent assay, are accurate and effective, but are greatly limited because they are complex and time-consuming. Electrochemiluminescence (ECL) biosensors combine the advantages of both electrochemical and photoluminescence analysis and are suitable for high sensitivity and simple pathogenic bacteria detection. In this review, we summarize recent advances in ECL sensors for pathogenic bacteria detection and highlight the development of paper-based ECL platforms in point of care diagnosis of pathogens.
Highlights
Pathogenic bacteria, such as Staphylococcus aureus (S. aureus), Salmonella typhimurium (S. typhimurium), Escherichia coli (E. coli) O157:H7 and Listeria monocytogenes (L. monocytogenes), etc., are great threats to human health and safety [1,2]
Raw materials and any processed foods may be contaminated by pathogenic bacteria
Culture-independent biosensors can be viewed as cutting-edge technology since they offer transcendental advantages for the detection of bacteria, such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR); both of them can be combined with fluorescence, electrochemical, surface enhanced Roman scattering (SERS) and colorimetric assays [8,9,10,11,12] to quantify the pathogen concentrations
Summary
Pathogenic bacteria, such as Staphylococcus aureus (S. aureus), Salmonella typhimurium (S. typhimurium), Escherichia coli (E. coli) O157:H7 and Listeria monocytogenes (L. monocytogenes), etc., are great threats to human health and safety [1,2]. Culture-independent biosensors can be viewed as cutting-edge technology since they offer transcendental advantages for the detection of bacteria, such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR); both of them can be combined with fluorescence, electrochemical, surface enhanced Roman scattering (SERS) and colorimetric assays [8,9,10,11,12] to quantify the pathogen concentrations. The unstable excited state turns into the ground state and emits photos, according to the way free radicals are produced in ECL. It divides the quenching pathway and the co-reaction pathway [41]
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