Recent Advances in Detection, Isolation, and Imaging Techniques for Sulfane Sulfur-Containing Biomolecules.

Kenjiro Hanaoka,Honami Echizen,Eita Sasaki

doi:10.3390/biom11111553

Kenjiro Hanaoka, Honami Echizen + Show 1 more

Open Access

https://doi.org/10.3390/biom11111553

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Journal: Biomolecules	Publication Date: Oct 20, 2021
Citations: 5	License type: CC BY 4.0

Affiliation: Keio University, The University of Tokyo

Abstract

Hydrogen sulfide and its oxidation products are involved in many biological processes, and sulfane sulfur compounds, which contain sulfur atoms bonded to other sulfur atom(s), as found in hydropersulfides (R-S-SH), polysulfides (R-S-Sn-S-R), hydrogen polysulfides (H2Sn), etc., have attracted increasing interest. To characterize their physiological and pathophysiological roles, selective detection techniques are required. Classically, sulfane sulfur compounds can be detected by cyanolysis, involving nucleophilic attack by cyanide ion to cleave the sulfur–sulfur bonds. The generated thiocyanate reacts with ferric ion, and the resulting ferric thiocyanate complex can be easily detected by absorption spectroscopy. Recent exploration of the properties of sulfane sulfur compounds as both nucleophiles and electrophiles has led to the development of various chemical techniques for detection, isolation, and bioimaging of sulfane sulfur compounds in biological samples. These include tag-switch techniques, LC-MS/MS, Raman spectroscopy, and fluorescent probes. Herein, we present an overview of the techniques available for specific detection of sulfane sulfur species in biological contexts.

Highlights

Hydrogen sulfide (H2 S) was shown to be a neuromodulator in the brain in 1996 [1], and since many biological studies have established that H2 S is an endogenously produced gasotransmitter that is involved in multiple physiological processes, including cytoprotection against oxidative stress, mediation of neurotransmission, and regulation of inflammation [2]
We examined the reactivity of 2-thio RB with sulfane sulfurs in the presence of a biologically relevant reductant, GSH, at the concentration of 5 mM, since GSH is reported to be present at the mM level in living cells [43]
The results suggested that hydrogen polysulfides may be synergistically generated by the interaction of H2 S and nitric oxide (NO) to activate

Summary

Introduction

Hydrogen sulfide (H2 S) was shown to be a neuromodulator in the brain in 1996 [1], and since many biological studies have established that H2 S is an endogenously produced gasotransmitter that is involved in multiple physiological processes, including cytoprotection against oxidative stress, mediation of neurotransmission, and regulation of inflammation [2]. The strong electrophilicity of MBB may be problematic because it may lead to decomposition of the polysulfide adducts during the alkylation process [25] Related to this issue, sulfide detection with MBB in biological samples such as human blood serum and plasma requires special caution because MBB irreversibly consumes free sulfide and continues to extract sulfide from other reversibly bound sulfide complexes [26]. Lead to decomposition of the polysulfide adducts during the alkylation process [25]

Raman Imaging

Fluorescent Probes

Conclusions