Abstract

Imperative utilization of biosensors has acquired paramount importance in the field of drug discovery, biomedicine, food safety standards, defense, security, and environmental monitoring. This has led to the invention of precise and powerful analytical tools using biological sensing element as biosensor. Glucometers utilizing the strategy of electrochemical detection of oxygen or hydrogen peroxide using immobilized glucose oxidase electrode seeded the discovery of biosensors. Recent advances in biological techniques and instrumentation involving fluorescence tag to nanomaterials have increased the sensitive limit of biosensors. Use of aptamers or nucleotides, affibodies, peptide arrays, and molecule imprinted polymers provide tools to develop innovative biosensors over classical methods. Integrated approaches provided a better perspective for developing specific and sensitive biosensors with high regenerative potentials. Various biosensors ranging from nanomaterials, polymers to microbes have wider potential applications. It is quite important to integrate multifaceted approaches to design biosensors that have the potential for diverse usage. In light of this, this review provides an overview of different types of biosensors being used ranging from electrochemical, fluorescence tagged, nanomaterials, silica or quartz, and microbes for various biomedical and environmental applications with future outlook of biosensor technology.

Highlights

  • The term “biosensor” refers to powerful and innovative analytical device involving biological sensing element with wide range of applications, such as drug discovery, diagnosis, biomedicine, food safety and processing, environmental monitoring, defense, and security

  • The present review is divided to various subsections describing two major technical strategies followed by various types of biosensor devices ranging from electrochemical, optical/ visual, polymers, silica, glass, and nanomaterials

  • We reviewed the different types of biosensors and their applications

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Summary

Frontiers in Bioengineering and Biotechnology

Imperative utilization of biosensors has acquired paramount importance in the field of drug discovery, biomedicine, food safety standards, defense, security, and environmental monitoring. This has led to the invention of precise and powerful analytical tools using biological sensing element as biosensor. Integrated approaches provided a better perspective for developing specific and sensitive biosensors with high regenerative potentials. Various biosensors ranging from nanomaterials, polymers to microbes have wider potential applications. This review provides an overview of different types of biosensors being used ranging from electrochemical, fluorescence tagged, nanomaterials, silica or quartz, and microbes for various biomedical and environmental applications with future outlook of biosensor technology

INTRODUCTION
TECHNICAL STRATEGIES
ELECTROCHEMICAL BIOSENSORS
GENETICALLY ENCODED OR SYNTHETIC FLUORESCENT BIOSENSORS
TECHNOLOGICAL COMPARISON OF BIOSENSORS
Silicon biosensor
Genetically encoded Fluorescence or fluorescencetagged biosensor
Cancer biomarker development and applications
Protein biology
BIOSENSOR TECHNOLOGY

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