Recent Advances and Best Practices for Cryo-EM Analysis for Protein Structure Determination

Abstract

ARTICLES DISCUSSED: de Martin Garrido, N., Ramlaul, K., Aylett, C. H. S. Preparation of sample support films in transmission electron microscopy using a support floatation block. Journal of Visualized Experiments. (170), doi:10.3791/62321 (2021). Klebl, D. P., Sobott, F., White, H. D., Muench, S. P. Fast grid preparation for time-resolved cryo-electron microscopy. Journal of Visualized Experiments. (177), doi:10.3791/62199 (2021). Budell, W. C., Allegri, L., Dandey, V., Potter, C. S., Carragher, B. Cryo-Electron microscopic grid preparation for time-resolved studies using a novel robotic system, Spotiton. Journal of Visualized Experiments. (168), doi:10.3791/62271 (2021). Nguyen, H. P. M., McGuire, K. L., Cook, B. D., Herzik, M. A., Jr. Manual blot-and-plunge freezing of biological specimens for single-particle cryogenic electron microscopy. Journal of Visualized Experiments. (180), doi:10.3791/62765 (2022). Martynowycz, M. W., Gonen, T. Microcrystal electron diffraction of small molecules. Journal of Visualized Experiments. (169), doi:10.3791/62313 (2021). Bisson, C., Hecksel, C. W., Gilchrist, J. B., Fleck, R. A. Preparing lamellae from vitreous biological samples using a dual-beam scanning electron microscope for cryo-electron tomography. Journal of Visualized Experiments. (174), doi:10.3791/62350 (2021). Wypych, D., Kierecki, D., Golebiowski, F. M., Rohou, A. gP2S, an information management system for CryoEM experiments. Journal of Visualized Experiments. (172), doi:10.3791/62377 (2021).