Abstract
We present a quantitative analysis of the deviations introduced by the anisotropy of the fluorescence on the measurement of the quantum yield of emission. Errors of up to 40% can be encountered depending on the characteristics of both sample and apparatus. Additionally, we have analyzed the effect of anisotropy on the study of fluorescence quenching in Stern-Volmer experiments, and on the reconstruction of time-resolved emission spectra. We conclude that the use of polarizers is recommended provided they are well characterized.
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