RecA-dependent increased precise excision of Tn10 in Salmonella typhimurium

Abstract

UV irradiation induced the precise excision of Tn10 inserted in met, trp or srl in a Salmonella tryphimurium strain; mitomycin C was also found to induced the frequency of precise excision of Tn10 from srl or met.Precise excision of Tn10 was not increased by either UV or mitomycin C in a recA mutant. Similarly, a recA mutant derived from uvrD strain showed a drastic reduction in the high spontaneous levels of precise excision of Tn10 of this strain. These results indicate that recA is involved in the increased precise excision of Tn10.In contrast to point mutations excision of Tn10 was found of Tn10 was found to be UV inducible in a top mutant.