Abstract
Surveillance for influenza virus in pigs in the United Kingdom during spring 2010 detected a novel reassortant influenza virus. This virus had genes encoding internal proteins from pandemic (H1N1) 2009 virus and hemagglutinin and neuraminidase genes from swine influenza virus (H1N2). Our results demonstrate processes contributing to influenza virus heterogeneity.
Highlights
A/swine/ England/1382/10 had HA and NA genes closely related to UK swine subtype H1N2 viruses (Table 1)
We report detection of a novel reassortant virus between pandemic (H1N1) 2009 virus and a swine subtype H1N2 virus
In contrast to an earlier report of a reassortant virus that contained the NA gene of pandemic (H1N1) 2009 virus (8), in this study all genes encoding internal proteins of A/swine/England/1382/10 virus are derived from pandemic (H1N1) 2009 virus
Summary
M, and nonstructural genes) and pandemic (H1N1) 2009 virus–specific primers (PB2, PB1, acidic polymerase, and NP genes). A/swine/ England/1382/10 had HA and NA genes closely related to UK swine subtype H1N2 viruses (Table 1). All genes encoding internal proteins showed the highest similarity to pandemic (H1N1) 2009 viruses (Table 1). The M gene of A/swine/England/1382/10 had the S31N amantadine-resistance mutation, typical of pandemic (H1N1) 2009 viruses. It had 627E and 701D mutations in the PB2 gene and mutation 591R, a basic amino acid that reportedly compensates for lack of the 627K mammalian-adaptive mutation (14). Solid diamonds indicate A/swine/England/1382/10 genes from virus isolated in this study, and open diamonds indicate genes from other viruses reported in this study. The 10 acute-phase and 9 convalescent-phase serum samples were subjected to standard HI tests (10) with antigens from A/swine/England/195852/92 (avian-like subtype H1N1), A/swine/England/1353/09 pandemic (H1N1) 2009 virus, A/swine/England/438207/94 (subtype H1N2), and homologous A/swine/England/1382/10 (Table 2). Antibody titers to endemic and reassortant subtype H1N2 viruses were negligible in acute-phase serum samples but increased 14-fold and 16-fold, respectively, in convalescent-phase serum samples
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
